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_INLET.INF / _HISTORY.INF

Auxiliary files found in some Waters RAW directories. Neither is required for spectrum decoding.

_INLET.INF

Format: Plain text (ACE Experimental Record)

A human-readable ASCII text file generated by the Waters ACE (Automated Chromatographic Experiment) software. Contains the LC method parameters used for the run.

Corpus presence

DatasetPresentNotes
PXD058812 (P15, Q-TOF native MS)NoNo LC inlet
PXD066594 (WANG, SYNAPT G2-Si IMS)NoNative MS, no LC
PXD068881 (CtpA, SYNAPT G2-Si, LC-MS)Yes4358 bytes
PXD075602 (DHPR, Xevo G2-XS, LC-MS)Yes4511 bytes

Only present for LC-MS acquisitions that used a Waters inlet method.

Content

The file begins with the header line:

\r\nACE Experimental Record\r\n

followed by sections describing:

  • Inlet method file path
  • Run method parameters
  • Pump configuration (Waters BSM or other binary solvent manager)
  • Solvent composition, flow rate, gradient table
  • Column parameters
  • Sampler/autosampler settings

Example excerpt (DHPR):

ACE Experimental Record

Inlet Method File: d:\projects\mpd002.pro\acqudb\20231012_traping_5ul_2min_trap_30min

--------------------- Run method parameters ----------------

-- PUMP --

Waters GI Pump
...
Binary Solvent Manager
...

This file is informational only and is not required for spectrum data decoding.


_HISTORY.INF

Format: Waters Parameter Table (binary, 0 descriptors)

A binary file using the Waters Parameter Table structure (see 07-func-sts.md for format description) with 0 channel descriptors.

Corpus presence

DatasetPresentSize (bytes)VersionData bytes
PXD058812 (P15, Q-TOF)Yes6243592
PXD066594 (WANG, SYNAPT G2-Si)Yes1731121699
PXD068881 (CtpA, SYNAPT G2-Si)No---
PXD075602 (DHPR, Xevo G2-XS)No---

Preamble

u16@0 = data_offset (always 32 = 32 + 0*48; no descriptors)
u16@2 = version (3 for P15; 12 for WANG)
u16@4 = record_size (0 for P15; 1 for WANG)
u16@6 = n_desc (0 in all observed cases)

The preamble is valid Waters Parameter Table format, but with n_desc=0 there are no channel descriptors. The data section follows immediately at byte 32.

Data section

Content varies by dataset and is not decoded:

  • P15 (592 bytes): begins with ~100 zero bytes, followed by float-like binary data (values in the range 0.1-4.0 based on byte pattern inspection); possibly an audit trail of parameter settings or a time-series of instrument state.
  • WANG (1699 bytes): rec_sz=1 suggests single-byte records; content not analyzed.

The HISTORY file likely records parameter changes made during the acquisition (e.g. autosampler injections, operator modifications). Its content is not needed for spectrum reconstruction.